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Exosome capture and isolation Kits is designed and optimized for isolation and enrichment of distinct sub-populations of exosomes from cell culture media or urine. Exosomes are extracellular vesicles secreted by most cell types and contain some microRNAs and surface marker proteins such as CD9, CD63, CD81. The kit consists of: 1um magnetic Capture Beads coupled with antibodies that recognize exosome surface antigens CD9, CD63, CD81, and Washing/Dilution Buffer and Exosome Elution Buffer. The kit uses functionalized magnetic microparticles (Capture Beads) for exosome capture. The beads are modified with hydrophilic polymers to decrease non-specific binding and conjugated with antibody, which specifically binds to antigen of exosomes. The captured exosomes can be directly applied for Western Blot, Flow Cytometry and qRT-PCR etc, or gently eluted from magnetic beads for applications such as NTA, Transmission Electron Microscopy and Labeling& Tracking.